The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand.
Reviews of earlier editions:
"good practical advice that is presented in a pleasantly readable form" Analytical Biochemistry
"well organized and written clearly" American Scientist
"should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" Nature
A guide providing a brief account of the main protein fractionation methods, with some simple theoretical and thermodynamic explanations of the events occurring, for students and researchers involved in the process of isolating an enzyme, from whatever source. A basic background in biochemistry and protein chemistry is assumed. This updated edition (2nd ed., 1987) adds new material on purification of special protein types (membrane proteins, recombinant proteins, antibodies) and on affinity chromatography. Annotation c. Book News, Inc., Portland, OR (booknews.com)