Fundamentals of Light Microscopy and Electronic Imaging
Douglas B. Murphy
Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscopeskills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image.
Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as:
Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions.
Both experienced and novice microscopists will find Fundamentals of Light Microscopy and Electronic Imaging an essential resource.
Reviewer:Alvin Telser, PhD(Northwestern University Feinberg School of Medicine)
Description:This book on the use of the light microscope introduces all of its classical and modern techniques and applications. It provides thorough theoretical and practical explanations and examples of how to use a microscope in all these applications.
Purpose:The author intends this book to be used by beginners in light microscopy and has written it with that emphasis. This is a very worthwhile purpose since there have been very few, or perhaps, no textbooks of this type in many years.
Audience:Although this book is intended to be used by beginners, it would be useful to more experienced microscopists as well because of the range of topics and the expertise, clarity, and thoroughness with which they are covered. The author of this book is a well known cell biologist and a recognized expert in the many applications of light microscopy.
Features:This book has 16 chapters, three appendixes and a glossary. The chapters describe and discuss essentially all of the modern uses of light microscopy, including the use and role of digital technology. Each chapter provides a theoretical basis for that type of microscopy, practical instructions on how to set up the microscope for that kind of optics, and useful ways of demonstrating the technique to the learner. The appendixes, glossary, and index are thorough and useful.
Assessment:This excellent book is not only well written, useful, and informative, it fills a gap in the textbook literature on the fundamentals of light microscopy. It will be of use in many cell biology courses and as a reference book for anyone who wishes to use any technique of light microscopy.